Regulatory
Part:BBa_K2782006:Design
Designed by: Bing Gao Group: iGEM18_BUCT-China (2018-08-16)
Synthetic repressor DNA sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 654
Design Notes
Note the addition of a cleavage site that may be used before and after the sequence.
Source
This sequence is derived from the glyoxylate operon (promoter) and is part of it. From Escherichia coli strain C600 chromosome, complete genome Sequence ID: CP031214.