Regulatory

Part:BBa_K2782006:Design

Designed by: Bing Gao   Group: iGEM18_BUCT-China   (2018-08-16)


Synthetic repressor DNA sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 654


Design Notes

Note the addition of a cleavage site that may be used before and after the sequence.


Source

This sequence is derived from the glyoxylate operon (promoter) and is part of it. From Escherichia coli strain C600 chromosome, complete genome Sequence ID: CP031214.


References